DNA

Part:BBa_K524203:Design

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST   (2012-08-26)

pSC101 heat sensitive origin of replication with deletion in par locus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1709
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The par locus was deleted through excision of BBa_K524000 by SspI and StuI. The resulting product generated 3 fragments with blunt ends. One of the fragments contained the par locus and some junk sequences. The three fragments were resolved with gel electrophoresis and only the two larger fragments with the par locus were recovered. Upon ligation, some plasmids had resulted in this new BioBrick in the orignal pSB1C3 vector.

Source

Modified from BBa_K524000

References

Tucker WT, Miller CA, Cohen SN. Structural and functional analysis of the par region of the pSC101 plasmid. Cell. 1984;38:191–201.